In Vivo Targeting of Escherichia coli with Vancomycin-Arginine

Arginine Decarboxylase from Escherichia coli

A total of three pyridoxyl-peptides were isolated from the inducible argmine decarboxylase of Escherichia coli B following reduction with NaBH4 and proteolysis with trypsin or chymotrypsin. Sodium borohydride reduces the Schiff base formed between pyridoxal-5’-P and the e-amino group of a lysyl residue of the protein. The sequence analysis of the three peptides is consistent with a unique pyrid...

Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli.

Arginine catabolism produces ammonia without transferring nitrogen to another compound, yet the only known pathway of arginine catabolism in Escherichia coli (through arginine decarboxylase) does not produce ammonia. Our aims were to find the ammonia-producing pathway of arginine catabolism in E. coli and to examine its function. We showed that the only previously described pathway of arginine ...

cloning of arginine deiminase from pseudomonas aeruginosa in escherichia coli

Biosynthetic Arginine Decarboxylase from Escherichia coli

Biosynthetic arginine decarboxylase of Escherichia coli has been purified 1,500-fold. The purified enzyme showed two bands on disc gel electrophoresis, both carrying out the decarboxylation of L-arginine. Electrophoresis in the presence of sodium dodecyl sulfate and sedimentation equilibrium ultracentrifugation was consistent with the enzyme being a tetramer of approximately 296,000 molecular w...

Control of Uracil Synthesis by Arginine in Escherichia Coli.

Ben-Ishai, Ruth (Israel Institute of Technology, Haifa), Michal Lahav, and Ada Zamir. Control of uracil synthesis by arginine in Escherichia coli. J. Bacteriol. 87:1436-1442. 1964.-It is shown that arginine affects uracil biosynthesis in Escherichia coli. The effect of arginine on uracil synthesis was analyzed by following the changes in the level of aspartate transcarbamylase, the first specif...